Production of a monoclonal antibody in a heterokaryon fungus or a fungal host Cell

摘要

Relates to methods for producing a monoclonal antibody in a heterokaryon fungus or in a fungal host cell.In addition, it also refers to a construction of nucleic acid, a First Nucleic Acid Sequence encoding a light chain of Antibody, and a Third Nucleic Acid Sequence encoding a heterologous Peptide Signalling to the first Sequence at Nucleic Acid,Construction of a Nucleic Acid comprising a First Nucleic Acid Sequence encoding a heavy chain Antibody and a third of a Nucleic Acid Sequence encoding a heterologous Peptide Signalling the first Nucleic Acid Sequence.Construction of a Nucleic Acid comprising a First Nucleic Acid Sequence encoding a light chain of Antibody and a Second Nucleic Acid Sequence encoding a Cellulose binding domain.Construction and a Nucleic Acid which includes a First Nucleic Acid Sequence encoding a heavy chain Antibody and a Second Nucleic Acid Sequence encoding a Cellulose binding domain.Claim 1: a method for producing a monoclonal antibody that includes: a) providing a heterokaryon fungus that includes a First Core and the second Core, wherein the first Core includes a First Construction of nucleic acid which includes a First Nucleic Acid Sequence. And encodes for a light chain of antibodyAnd wherein Said Second Core includes a Second Construction of nucleic acid which includes a First Nucleic Acid Sequence encoding a heavy chain Antibody.And where at least one of said Nucleic Acid constructs also comprises a Second Nucleic Acid Sequence encoding a polypeptide or a functional part of the normally secreted by a fungus; (b) cultivate the heterokaryon fungus under appropriate conditions. The expression of the Light And Heavy Chains of the Antibody.