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PROCESS FOR OBTAINING A BIOACTIVE CONCENTRATE WITH ANTIHYALURONIDASE ACTIVITY, FROM SMALL SEA FISH
PROCESS FOR OBTAINING A BIOACTIVE CONCENTRATE WITH ANTIHYALURONIDASE ACTIVITY, FROM SMALL SEA FISH
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机译:从小海鱼获得具有抗透明质酸酶活性的生物活性浓缩物的方法
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摘要
the process according to the invention consists in that the marine fish petty shredding, treated with a solution of purified water, fenolatu0103, concentration of 0,5% or with purified water, containing up to 1% complex based on diazolinidylurea, methylparaben, propylparaben and propilengli col - or solution of water - activated structured inhibitor, containing 1% diazolinidylurea, methylparaben,propylparaben and propylene glycol or with purified water acidic up to 1% ascorbic acid and 0.5% phenol, or water - activated inhibitor structured, sparkling with up to 1% ascorbic acid and 0.5% phenol, or with purified water containing up to 2,5% benzyl alcohol, the content of marine fish petty and solvent extraction being 1: 5.5.5, is carried out by maceration extraction for 48 h, at a temperature of 2...5u00b0c, stirring in the first 36 h, for 3 min, 3 h, and in the last 12 h, the mixture is stirred to allow sedimentation fish tissue, after which the extract obtained is filtered, is co vacuum ncentreazu0103 maximum 36u00b0c, until a report of concentration of 15: 1, leave to stand for 10 12 h, 2...5, centrifuge, deproteinizeazu0103 with ethanol 96c in ratio of 1: 2.4, stirring stirring for 3 min and then stand at 2...5u00b0c, filter through filter paper, industrial, is treated in order to obtain the content of 9...13% in dry matter with doubly distilled water and phenol, until a concentration of 4% phenol in extract, or only with doubly distilled water, in case of extraction solvents with purified water, methylparaben, propylparaben with diazolinidylurea, and propylene glycol or the booze riddled
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