The invention concerns a preparation of micro-organisms immobilised in beads acclimated to alcohol and/or acidity, and partly dried, capable of developing a fermenting activity when they are introduced in alcohol or acid musts. The invention also concerns a method for obtaining such a preparation comprising an acclimating step and an immobilising step for the micro-organisms, said steps being carried out in any sequence, but necessarily preceding a partial dehydrating step. The invention provides the advantages on enabling the micro-organisms to be preserved in viable and active form for several months, and to be used directly for reactivating interrupted fermenting processes, in particular alcoholic fermentation processes, for producing wine and other fermented beverages.
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