diagnostic procedures using the expression of mn / ca9 protein in agus - pap and

• 1. 美洲商陆抗病毒蛋白(PAP)在巴斯德毕赤酵母中的表达方法 [P] . 中国专利： CN1724673A . 2006-01-25
• 2. 重组猪细小病毒VP2蛋白、表达该蛋白的重组多角体病毒及其在疫苗制备和病毒诊断中的应用 [P] . 中国专利： CN103936839B . 2016.11.30
• 3. diagnostic procedures using the expression of mn / ca9 protein in agus - pap and [P] . 德国专利： DE60029140D1 . 2006-08-10

  机译： 诊断程序使用mn / ca9蛋白在琼脂中的表达-pap and

• 4. DIAGNOSTIC METHOD USING EXPRESSION OF MN/CA9 PROTEIN IN AGUS PAP SMEARS [P] . 欧洲知识产权局专利： EP1261641B1 . 2006-06-28

  机译： 利用MN / CA9蛋白在阿格氏涂片涂片中表达的诊断方法

• 5. DIAGNOSTIC METHOD USING EXPRESSION OF MN/CA9 PROTEIN IN AGUS PAP SMEARS [P] . 欧洲知识产权局专利： EP1261641A4 . 2005-06-15

  机译： 利用MN / CA9蛋白在阿格氏涂片涂片中表达的诊断方法

• 1. Susceptibility to AcMNPV and Expression of Recombinant Proteins by a Novel Cell Clone Derived from a Trichoplusia ni QAU-BTI-Tn9-4s Cell Line [J] . Ming Shan, Shi-ying Zhang, Lei Jiang, 中国病毒学：英文版 . 2011,第005期
• 2. Tissue protein expressions of human lung carcinomas and in the application of clinical diagnostics at the protein level [C] . (missing) International Beijing conference and exhibition on instrumental analysis . 2003
• 3. APE1/Ref-1 Redox Signaling Regulates HIF1a-Mediated CA9 Expression in Hypoxic Pancreatic Cancer Cells: Combination Treatment in Patient-Derived Pancreatic Tumor Models [D] . Logsdon, Derek Paul. 2018
• 4. Hypomethylation of the MN/CA9 promoter and upregulated MN/CA9 expression in human renal cell carcinoma [O] . M Cho, H Uemura, S-C Kim, 2001
• 5. Hypomethylation of the MN/CA9 promoter and upregulated MN/CA9 expression in human renal cell carcinoma [O] . M Cho, H Uemura, S-C Kim, 2001

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Effects of minimally invasive procedures for removal of intracranial hematoma on matrix metalloproteinase expression and blood-brain barrier permeability in perihematomal brain tissues [J] . . 中华神经外科疾病研究杂志 . 2010,第6期
• 3. Potential of proteins and their expression level in marine phytoplankton (iPrymnesium parvum/i) as biomarker of N, P and Fe conditions in aquatic systems [J] . Hiroshi Hasegawa ,M. Mamunur Rahman ,Sayaka Kato . 生物化学进展（英文） . 2013,第3期
• 4. Susceptibility to AcMNPV and Expression of Recombinant Proteins by a Novel Cell Clone Derived from a Trichoplusia ni QAU-BTI-Tn9-4s Cell Line [J] . Ming Shan ,Shi-ying Zhang ,Lei Jiang . 中国病毒学 . 2011,第005期
• 5. HBHA蛋白缺失端表达纯化及其在结核病临床诊断中应用研究 [C] . 周珊 ,徐修礼 ,马越云 . 2012年中华医学会全国临床微生物学术交流大会 . 2012
• 6. 免疫组化五项指标(PSA、PAP、HCK、P63、AMACR)的表达在前列腺病变诊断中的应用价值 [A] . 曹亮 . 2010