METHOD FOR IN VITRO MOLECULAR EVOLUTION OF A PROTEIN FUNCTION.

摘要

A method for generating a sequence or a population of polynucleotide sequences from single-stranded stem polynucleotide sequences encoding one or more protein motifs, comprising the steps of: a) providing single-stranded DNA that constitutes strands of more and less of the mother polynucleotide sequences; b) digest the single stranded polynucleotide sequences with an exonuclease to generate populations of single strand fragments; c) contacting said fragments generated from the strands more with fragments generated from the strands less and optionally, adding the template sequences that are fused to terminals 3 '' and 5 '' of at least one of the mother polynucleotides under conditions of fusion; d) amplifying the fragments that are fused together to generate at least one polynucleotide sequence encoding one or more protein motifs having altered characteristics compared to the one or more protein motifs encoded by said parent polynucleotides.