Novel method for integrating a foreign DNA into eukaryotic genomes.

摘要

recombinant protein comprising: a first site-specific recombinase fused in frame with a second distinct site-specific recombinase. compositions and methods for introducing a DNA of interest into a genomic integration site are provided. In particular, the methods and compositions involve the use of a combination of target sites two different specific recombinases, such as Cre and FLP site, and expression of a chimeric recombinase with dual target site specificity. Thus, the compositions comprise novel site-specific recombinases with specificities to multiple target sites compared, and nucleotide sequences and expression cassettes encoding these recombinases or target to sites. The methods involve transforming a eukaryotic cell having target sites for the novel recombinase with DNA of interest that is flanked by corresponding target sites. Or expression of novel or two site-specific recombinases in the eukaryotic cell a chimeric recombinase results in the integration of the DNA interest into the genome. The compositions and methods of the invention make use in the construction of eukaryotic cells stably transformed, and in particular plant cells.