SELECTION OF MICROORGANISMS WITH GROWTH DEPENDENT UPON EXTRACYTOPLASMIC ENZYMES

摘要

A method for selection of microorganisms with improved growth on non-native substrates is disclosed. Strains expressing tethered, enzymes on their cell surface are grown on non-native substrates and mutants with improved growth characteristics relative to the parent strain can be advantageously selected. Both soluble and insoluble substrates may be employed.