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NARROW I.D. MONOLITHIC CAPILLARY COLUMNS FOR HIGH EFFICIENCY SEPARATION AND HIGH SENSITIVITY ANALYSIS OF BIOMOLECULES
NARROW I.D. MONOLITHIC CAPILLARY COLUMNS FOR HIGH EFFICIENCY SEPARATION AND HIGH SENSITIVITY ANALYSIS OF BIOMOLECULES
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机译:窄行用于生物分子的高效分离和高灵敏度分析的单色谱毛细管柱
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摘要
A method of preparing an ultra-nanoscale-LC monolithic separation medium for use in capillary columns, or channels in microfabricated devices (microchips), and capillaries prepared by the method are disclosed. The application of moderate positive pressure to both ends of the capillary during the monolith polymerization process permits the preparation of monolithic capillary columns having very low i.d., e.g., 25 μm and smaller, with enhances mass transfer properties and low back pressures, and excellent column-to-column reproducibility of retention times.
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