METHODS FOR PORLIFERATION OF HEMATOPOIETIC STEM/PROGENITOR CELL IN VITRO

摘要

The present invention relates to methods for proliferation of hematopoietic stem/progenitor cells in vitro. Feeder cells with Wnt3a gene introduced into can be used as cultured layers in the methods provided by the invention. Wnt3a gene may be introduced into and expressed in feeder cells, and it is proved in this study that the feeder layers secrete the active Wnt3a protein in natural form. Compared to directly adding purified Wnt3a protein, the methods of the present invention allow more stable effect of proliferation, more convenient application and less cost. A large scale of proliferation in vitro of hematopoietic stem cells may be performed, which may benefit the solution to the problem interfering the clinic hematopoietic stem cell transplant, i.e. the lack of cell donors.