PROTEIN CHIPS, METHOD PRODUCING IT AND DETECTION SYSTEM OF THE PROTEIN CHIPS, AND OPERATING METHOD OF THE DETECTION SYSTEM

摘要

The invention is related to protein chips, the methods producing it, the use thereof, wherein the protein chip can operate multiple index detection in parallel. The protein chip comprises a solid carrier and proteins being detectable multiple index in parallel, the various of proteins are fixed on the carrier in order by automatic sample system. The parts that does not drop samples on the chip are blocked by blocking solution, lyophilized and stored. The invention is also related to the detection system of the protein chip above, comprising protein chip, mixture of various labelled proteins with peroxidase, diluent, chemical composition that can perform chemoluminescence reaction, detergent and standard. The principle is: contacting the solid carrier combined with various proteins A that can be specially binding substance in vivo with sample solutions containing target proteins B, forming stable complex A-B, further reaction with the corresponding labelled AC-labelled specially binding protein to form stable A-B-C- labelled, in which the label is a chemoluminescence marker that can give detectable signal. The protein chip can significantly improve detection accuracy, and can advantageously give high reliability and detection sensibility. The protein chips are broadly useful in areas of molecular biology, bio-medicine, human proteinic study, especially clinical diagnosis.