MONOCLONAL ANTIBODY SPECIFIC TO DENATURED HUMAN LEUKOCYTE ANTIGEN CLASS I

摘要

[PROBLEMS] To provide a monoclonal antibody capable of simultaneously detecting all the expression of three kinds of heavy chain proteins, HLA-A, HLA-B, and HLA-C that constitute human leukocyte antigen class I (HLA class I) using a denatured human tissue such as a specimen fixed with formalin or the like as a material. [MEANS FOR SOLVING PROBLEMS] It was found that an HLA class I specific antibody that preferentially binds to all the denatured HLA-A, -B and -C can be established by immunizing a mouse with a denatured recombinant HLA-A∗2404 heavy chain protein. The invention is directed to the monoclonal antibody that specifically binds to the respective heavy chains of HLA-A, HLA-B and HLA-C of denatured human leukocyte antigen class I, which is produced by a hybridoma (FERM AP-20454) and specifically binds to the respective heavy chains of HLA-A, HLA-B and HLA-C of denatured human leukocyte antigen class I. Further, the invention is directed to a test agent for denatured human leukocyte antigen class I containing the monoclonal antibody as a primary component.