HIGH THROUGHPUT GLYCAN ANALYSIS FOR DIAGNOSING AND MONITORING RHEUMATOID ARTHRITIS AND OTHER AUTOIMMUNE DISEASES

摘要

A method for diagnosing and monitoring an autoimmune disease comprise releasing glycan of glycoproteins from samples of a body fluid without purifying the glycoproteins, and without exposing the body fluid to hydrazinolysis; and quantitatively analyzing the glycans. Another method of diagnosing and monitoring an autoimmune disease comprise measuring diseased glycosylation profiles and one or more control glycosylation profiles, wherein the diseased glycosylation profiles are glycosylation profiles of glycans of glycoproteins from autoimmune disease patients and the one or more control glycosylation profiles are glycosylation profiles of glycans of glycoproteins from patients without the autoimmune disease; and comparing peak ratios in the diseased glycosylation profiles and in the one or more control glycosylation profiles and selecting a ration having a highest correlation with parameters of the autoimmune disease patients out of the peak ratios as a glycosylation marker of the autoimmune disease. A high throughput method for diagnosing and monitoring rheumatoid arthritis in a patient comprises releasing glycans of glycoproteins from a body fluid or a body tissue of the patient; and measuring a ratio between an amount of G0 glycans and an amount of G1 glycans in the glycans, wherein the G0 glycans and an amount of G1 glycans in the glycans, wherein the G0 glycans comprise no galactose and the G1 glycans comprise exactly one galactose.