ISOLATED CYTOTOXIC FACTOR ASSOCIATED WITH MULTIPLE SCLEROSIS AND METHOD OF DETECTING SAID CYTOTOXIC FACTOR

摘要

The invention relates to an isolated cytotoxic factor which is associated with multiple sclerosis and which is selected from heterocomplex GM2AP/GM2/MRP14 and mutated GM2AP/GM2/MRP14, and to the method of detecting said factor in a biological sample to be tested. The inventive method comprises the following steps consisting in: (i) bringing the biological sample into contact with (a) at least one capture antibody selected from antibodies that bind specifically to protein GM2AP, to the mutated GM2AP protein, to protein MRP14, to complex GM2AP/GM2, to the mutated GM2AP/GM2 complex and to complex MRP14/GM2, and (b) at least one labelled detection antibody selected from antibodies that bind specifically to protein GM2AP, to the mutated GM2AP protein, to protein MRP14, to complex GM2AP/GM2, to the mutated GM2AP/GM2 complex and to complex MRP14/GM2; and (ii) detecting and/or quantifying the cytotoxic factor by detecting and/or quantifying the labelled detection antibody.