PROBE OF HUMAN PAPILLOMAVIRUS, OLIGONUCLEOTIDE MICROARRAY AND GENOTYPING KIT COMPRISING THE SAME, AND GENOTYPING METHOD FOR HUMAN PAPILLOMAVIRUS USING THE SAME

摘要

The present invention is the major cause of cervical cancer and the most common sexual borne disease, human papilloma virus (human papillomaviruis, HPV) nucleic acid and complementary coupling probes, including DNA chip or microarray (chip or microarray), and using them to them to the that using HPV genotyping diagnostic kits, and it relates to a method for analyzing the infection and whether genotypes of HPV, of the general adult female 4898 people Pap specimens and 35 types in cervical cancer tissue samples of 68 patients in Korea HPV L1 and E6 / yielded a clone of the E7 gene, here by adding the material in Europe and America and synthesize oligonucleotide probes for analysis of HPV 40-type, were produced DNA chip using these probes, a variety of clinical specimens the analysis has been completed by the value of the DNA chip. The DNA chip may know exactly both the HPV 40-types found in the cervix, may diagnose complex infection by more than one HPV type-specific and diagnostic sensitivity of the HPV genotype also all close to 100% high, also high reproducibility, and to be able to scan a large number of samples in the shortest possible time and economically superior to conventional tests, it is useful for predicting cervical cancer and precancerous lesions to. ; Human papillomavirus (HPV), oligonucleotide microarray, DNA chip, PCR