EXPRESSION VECTOR INCLUDING NARK PROMOTER OR MUTATED NARK PROMOTER AND MASS-PRODUCTION METHOD OF ANY TARGET PROTEIN USING THE SAME

摘要

The present invention relates to a mass production method of the expression vector and the foreign protein using the same including a narK promoter, the -10 sequence of the promoter or the signal sequence of narK narK promoter represented by the number 1 TAAGGT substituted by a consensus sequence TATAAT a strain with the expression vector and the expression vector used as a promoter for the mass expression of the foreign protein to the narK promoter or a mutated narK promoter combines the DNA (foreign protein-encoding DNA) encoding a foreign protein to mutated narK promoter trait switches and provides a method for mass production with a foreign protein from the transformed strain. ; When using the expression vector according to the present invention, human growth hormone being much economic burden to the patients with the high cost of the manufacturing process up to now, interferon-beta, erythropoietin, prices for the therapeutic protein, such as granulocyte colony forming-stimulating factor expressed to induce and available, it can be supplied at a lower price to the afflicted patient's can significantly increase the expression level.