Plasmid DNA PSFE, encodes the synthesis of human epidermal growth factor, AND METHOD FOR PRODUCING with this DNA

摘要

1. u043fu043bu0430u0437u043cu0438u0434u043du0430u00a0 dna pSFE, u043au043eu0434u0438u0440u0443u044eu0449u0430u00a0 synthesis of human epidermal growth factor, u0445u0430u0440u0430u043au0442u0435u0440u0438u0437u0443u044eu0449u0430u00a0u0441u00a0 sukhoy of 1736.and u043du0435u0441u0443u0449u0430u00a0 the following elements: replication u043eu0440u0438u0434u0436u0438u043d pUC forces, gene of resistance to the u0430u043du0442u0438u0431u0438u043eu0442u0438u043au0443 u0430u043cu043fu0438u0446u0438u043bu043bu0438u043du0443, tac - promoter, u0441u0432u00a0u0437u0430u043du043du044bu0439 station landing lake - u0440u0435u043fu0440u0435u0441u0441u043eu0440 oh, and quickly built on the sites of restriction BsmI and Hind iii gene of hybrid protein SPEGF,u0441u043eu0441u0442u043eu00a0u0449u0435u0433u043e of human epidermal growth factor (egf) and the signal u043fu0435u043fu0442u0438u0434u0430 OmpA with edit sites u0443u0437u043du0430u0432u0430u043du0438u00a0 u0440u0435u0441u0442u0440u0438u043au0446u0438u043eu043du043du044bu043cu0438 enzymes BsmI and Hind iii, as well as the start and stop u043au043eu0434u043eu043du043eu0432, with gene SPEGF u043du0430u0445u043eu0434u0438u0442u0441u00a0 under u0440u0435u0433u0443u043bu00a0u0446u0438u0435u0439 tac - promoter or u0441u0432u00a0u0437u0430u043du043du043eu0433u043e station landing lake - u0440u0435u043fu0440u0435u0441u0441u043eu0440u0430 and fragment u043au043eu0434u0438u0440u0443u044eu0449u0438u043c station u0441u0432u00a0u0437u044bu0432u0430u043d u0438u00a0 u0440u0438u0431u043eu0441u043eu043c (rbs)to be successful u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 u0434u043bu00a0 protein in the cells of the bacteria. coli.;2. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 human epidermal growth factor (egf) through the expression of its gene in escherichia u0431u0430u043au0442u0435u0440u0438u00a0u0445. coli strain JM105, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, a synthetic gene, encrypting u0447u044du0444u0440, u043du0430u0445u043eu0434u0438u0442u0441u00a0 of u043fu043bu0430u0437u043cu0438u0434u043du043eu0439 dna pSFE, after the transformation of bacterial cells of the pla u0437u043cu0438u0434u043eu0439 and u043au0443u043bu044cu0442u0438u0432u0438u0440u043eu0432u0430u043du0438u00a0 u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 allocation u0447u044du0444u0440 of u043fu0435u0440u0438u043fu043bu0430u0437u043cu044b cells.