Plasmid DNA PBSH2EGF, encodes the synthesis of human epidermal growth factor, AND METHOD FOR PRODUCING with this DNA

摘要

1. u043fu043bu0430u0437u043cu0438u0434u043du0430u00a0 dna pBSH2EGF, u043au043eu0434u0438u0440u0443u044eu0449u0430u00a0 synthesis of human epidermal growth factor, u0445u0430u0440u0430u043au0442u0435u0440u0438u0437u0443u044eu0449u0430u00a0u0441u00a0 measuring 3393 sukhoy.and u043du0435u0441u0443u0449u0430u00a0 the following elements: quickly built-in restriction sites BamHI and Hind iii gene of human epidermal growth factor (u0447u044du0444u0440), which is built s start - and stop u043au043eu0434u043eu043du043eu0432, gene of resistance to the u0430u043du0442u0438u0431u0438u043eu0442u0438u043au0443 u043au0430u043du0430u043cu0438u0446u0438u043du0443, replication u043eu0440u0438u0434u0436u0438u043d pUC forces, gene, encrypting lake - u0440u0435u043fu0440u0435u0441u0441u043eu0440 and sequence u043au043eu0434u0438u0440u0443u044eu0449u0443u044e to okay u0441u0432u00a0u0437u044bu0432u0430u043du0438u00a0 u0440u0438u0431u043eu0441u043eu043c (rbs)the u0434u043bu00a0 started u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 protein in the cells of the bacteria u0435.Coli, with gene u0447u044du0444u0440 u043du0430u0445u043eu0434u0438u0442u0441u00a0 under u0440u0435u0433u0443u043bu00a0u0446u0438u0435u0439 t7 - promoter or u0441u0432u00a0u0437u0430u043du043du043eu0433u043e station landing lake - u0440u0435u043fu0440u0435u0441u0441u043e ra.;2. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 human epidermal growth factor through the expression of its gene in escherichia u0431u0430u043au0442u0435u0440u0438u00a0u0445. coli strain BL21 (DE3 u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0), so that a synthetic gene, encrypting u0447u044du0444u0440, u043du0430u0445u043eu0434u0438u0442u0441u00a0 of u043fu043bu0430u0437u043cu0438u0434u043du043eu0439 dna pBSH2EGF, after the transformation of bacterial cells danno in u043fu043bu0430u0437u043cu0438u0434u043eu0439 and u043au0443u043bu044cu0442u0438u0432u0438u0440u043eu0432u0430u043du0438u00a0 u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 allocation u0447u044du0444u0440 of u043du0430u043au0430u043fu043bu0438u0432u0430u044eu0449u0438u0445u0441u00a0 in cells bodies u0432u043au043bu044eu0447u0435u043du0438u00a0.