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Strains of the genus STREPTOMYCES, producing C-13 substituted NEMADEKTIN AND METHOD FOR C-13 substituted NEMADEKTINA USING THIS STRAIN
Strains of the genus STREPTOMYCES, producing C-13 substituted NEMADEKTIN AND METHOD FOR C-13 substituted NEMADEKTINA USING THIS STRAIN
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机译:链霉菌属的菌株,产生该C-13取代的内酰胺的菌株和使用该菌株的C-13取代的内酰胺的方法
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1. the strain of microorganism u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du043eu0433u043e subspecies Streptomyces cyaneogriseus and capable of producing with 13 u0433u043bu0438u043au043eu0437u0438u043bu0438u0440u043eu0432u0430u043du043du044bu0439 u043du0435u043cu0430u0434u0435u043au0442u0438u043d.;2. the strain of microorganism on p. 1, where the strain of microorganism capable of producing with 13 u0433u043bu0438u043au043eu0437u0438u043bu0438u0440u043eu0432u0430u043du043du044bu0439 u043du0435u043cu0430u0434u0435u043au0442u0438u043d, u00a0u0432u043bu00a0u0435u0442u0441u00a0 u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du044bu0439 subspecies Streptomyces cyaneogriseus nemA4:: vph attBTG1:: aveA4 - aveA3 - aveE attBC31:: aveR attBR4:: aveBI - BVIII (FERM bp - 8394).;3. the strain of microorganism u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du043eu0433u043e subspecies Streptomyces cyaneogriseus and capable of producing with 13 u0433u0438u0434u0440u043eu043au0441u0438u043bu043du0435u043cu0430u0434u0435u043au0442u0438u043d.;4. the strain of microorganism on p. 3, where the strain of microorganism capable of producing with 13 u0433u0438u0434u0440u043eu043au0441u0438u043bu043du0435u043cu0430u0434u0435u043au0442u0438u043d u00a0u0432u043bu00a0u0435u0442u0441u00a0 u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du044bu0439 subspecies Streptomyces cyaneogriseus nemA4:: vph attBTG1:: aveA4 - aveA3 - aveE attBC31:: aveR (FERM bp - 8395).;5. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 with 13 u0433u0438u0434u0440u043eu043au0441u0438u043bu043du0435u043cu0430u0434u0435u043au0442u0438u043du0430, which includes the cultivation of a strain of microorganism u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du043eu0433u043e subspecies Streptomyces cyaneogriseus capable of u043fu0440u043eu0434u0443u0446u0438u0440u043eu0432 down to - 13 u0433u0438u0434u0440u043eu043au0441u0438u043bu043du0435u043cu0430u0434u0435u043au0442u0438u043d in the environment, receiving and storing with - 13 u0433u0438u0434u0440u043eu043au0441u0438u043bu043du0435u043cu0430u0434u0435u043au0442u0438u043du0430 in u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 environment and selection of u043au0443u043bu044cu0442u0438u0432u0438u0440u0443u0435u043cu043e 13 u0433u0438u0434u0440u043eu043au0441u0438u043bu043du0435u043cu0430u0434u0435u043au0442u0438u043du0430 in the mass.;6. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 with 13 u0433u043bu0438u043au043eu0437u0438u043bu0438u0440u043eu0432u0430u043du043du043eu0433u043e u043du0435u043cu0430u0434u0435u043au0442u0438u043du0430, which includes the cultivation of a strain of microorganism u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du043eu0433u043e subspecies Streptomyces cyaneogriseus capable of p u0440u043eu0434u0443u0446u0438u0440u043eu0432u0430u0442u044c with 13 u0433u043bu0438u043au043eu0437u0438u043bu0438u0440u043eu0432u0430u043du043du044bu0439 u043du0435u043cu0430u0434u0435u043au0442u0438u043d in the environment, receiving and storing with - 13 u0433u043bu0438u043au043eu0437u0438u043bu0438u0440u043eu0432u0430u043du043du043eu0433u043e u043du0435u043cu0430u0434u0435u043au0442u0438u043du0430 in u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 environment and selection of 13 u0433u043bu0438u043au043eu0437u0438u043bu0438 u0440u043eu0432u0430u043du043du043eu0433u043e u043du0435u043cu0430u0434u0435u043au0442u0438u043du0430 of u043au0443u043bu044cu0442u0438u0432u0438u0440u0443u0435u043cu043eu0439 mass.;7. germ u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du043eu0433u043e subspecies Streptomyces cyaneogriseus containing group u0430u0433u043bu0438u043au043eu043du0430 biosynthesis genes u0430u0432u0435u0440u043cu0435u043au0442u0438u043du0430 Streptomyces avermitilis and capable of producing with 13 hydrox u0438u043bu043du0435u043cu0430u0434u0435u043au0442u0438u043d.;8. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 microorganism on p. 7 belonging to the u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du043eu043cu0443 u043fu043eu0434u0432u0438u0434u0443 Streptomyces cyaneogriseus containing group u0430u0433u043bu0438u043au043eu043du0430 biosynthesis genes u0430u0432u0435u0440u043cu0435u043au0442u0438u043du0430 Streptomyces avermitilis and capable of producing with 13 g u0438u0434u0440u043eu043au0441u0438u043bu043du0435u043cu0430u0434u0435u043au0442u0438u043d.;9. germ u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du043eu0433u043e subspecies Streptomyces cyaneogriseus containing group u0430u0433u043bu0438u043au043eu043du0430 biosynthesis genes u0430u0432u0435u0440u043cu0435u043au0442u0438u043du0430 Streptomyces avermitilis and capable of producing with 13 u0433u043bu0438u043au043eu0437u0438 u043bu0438u0440u043eu0432u0430u043du043du044bu0439 u043du0435u043cu0430u0434u0435u043au0442u0438u043d.;10. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 microorganism on p. 9 u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du043eu0433u043e subspecies Streptomyces cyaneogriseus containing group u0430u0433u043bu0438u043au043eu043du0430 biosynthesis genes u0430u0432u0435u0440u043cu0435u043au0442u0438u043du0430 Streptomyces avermitilis and capable of producing with 13 u0433u043bu0438u043au043eu0437u0438u043bu0438u0440u043eu0432u0430u043du043du044bu0439 u043du0435u043cu0430u0434u0435u043au0442u0438u043d.;11. the strain of microorganism u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du043eu0433u043e subspecies Streptomyces cyaneogriseus, u043fu0440u043eu0434u0443u0446u0438u0440u0443u044eu0449u0438u0439 u043du0435u043cu0430u0434u0435u043au0442u0438u043d, in which a gene of resistance to u0432u0438u043eu043cu0438u0446u0438u043du0443 area u043au043eu0434u0438u0440u0443u044eu0449u0435u0439 operas he KS10 genes nemA3 - 4 biosynthesis u0430u0433u043bu0438u043au043eu043du0430 u043du0435u043cu0430u0434u0435u043au0442u0438u043du0430 (u0438u043du0441u0435u0440u0446u0438u043eu043du043du044bu0439 mutant KS10).;12. the strain of microorganism on p. 11, where the strain of microorganism, which produced u043du0435u043cu0430u0434u0435u043au0442u0438u043d, u00a0u0432u043bu00a0u0435u0442u0441u00a0 u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du044bu0439 subspecies Streptomyces cyaneogriseus nemA4:: vph (FERM bp - 8393).;13. the strain of microorganism u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du043eu0433u043e subspecies Streptomyces cyaneogriseus containing u0438u043du0441u0435u0440u0446u0438u043eu043du043du043eu043c mute-o KS10 genes aveA3 - 4 biosynthesis u0430u0433u043bu0438u043au043eu043du0430 u0430u0432u0435u0440u043cu0435u043au0442u0438u043du0430 Streptomyces avermitilis and method flat to form a hybrid PKS based on NemA1 - 2 and AVES3 - 4.;14. the strain of microorganism u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du043eu0433u043e subspecies Streptomyces cyaneogriseus and capable to form hybrid PKS based on AVES3 NemA1 - 2 and 4, where the strain of microorganisms contains u0440u0435u0433u0443u043bu00a0u0442 u043eu0440u043du044bu0439 gene aveR biosynthesis genes u0430u0432u0435u0440u043cu0435u043au0442u0438u043du0430 Streptomyces avermitilis.;15. the strain of microorganism u043du0435u0446u0438u0430u043du043eu0433u0435u043du043du043eu0433u043e subspecies Streptomyces cyaneogriseus and capable to form hybrid PKS based on AVES3 NemA1 - 2 and 4, where the strain of microorganism contains u0440u0435u0433u0443u043bu00a0u0442u043e u0440u043du044bu0439 gene aveR biosynthesis genes and genes u0430u0432u0435u0440u043cu0435u043au0442u0438u043du0430 u0433u043bu0438u043au043eu0437u0438u043bu0438u0440u043eu0432u0430u043du0438u00a0 u0430u0432u0435u0440u043cu0435u043au0442u0438u043du0430 and biosynthesis u043eu043bu0435u0430u043du0434u0440u043eu0437u044b aveBI - BVIII Streptomyces avermitilis.
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