Strains with nemadectin producing ability belonging to the genus Streptomyces, manufacturing method of nemadectin using the strain

摘要

This invention alters the nemadekuchinagurikon biosynthesis gene group in order to be able as C-13 hidorokishirunemadekuchin to which the sugar can add nemadekuchin, produces the production stocks which produce C-13 hidorokishirunemadekuchin. And furthermore, distribution saccharification of ebamekuchin and me and introducing the avcBI-BVIII gene group which participates in roast biosynthesis, it produces C-13 gurikoshirunemadekuchin production stocks. This way, C-13 hidorokishirunemadekuchin and C-13 rank distribution can acquire nemadekuchin which is saccharified efficiently with the production stocks which are produced with molecular genetics technique the improvement of the biological activity can expect.