TRANSMISSIBLE GASTROENTERITIS OF SWINE VIRUS STRAIN FOR PRODUCTION OF DIAGNOSIS AND VACCINE PREPARATIONS

摘要

FIELD: veterinary virology and biotechnology.;SUBSTANCE: transmissible gastroenteritis of swine virus strain (TGSV) was isolated by sequential passages on SPEV (swine embryo kidney cell) and other cell cultures. Said virus strain is most effectively cultivated in 3-4-day monolayer of SPEV cells, obtained in roll-tube vessel, wherein after cultivation for 24-30 h infective activity level is 7.0-8.0 lg TCD₅₀/cm³. Strain survives starting characteristics for 18-month storage and for 9-10 passages (observation time) on SPEV cell culture.;EFFECT: TGSV strain with high biological, antigenic and immunogenic activity useful in production of high-specific and sensitive diagnosticums and high-immunogenic and harmless inactivated vaccine preparations.;7 tbl, 5 ex