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METHOD FOR PRODUCTION OF CITRIC ACID, ALPHA-AMYLASE AND GLUCOAMYLASE
METHOD FOR PRODUCTION OF CITRIC ACID, ALPHA-AMYLASE AND GLUCOAMYLASE
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机译:柠檬酸,α-淀粉酶和葡糖淀粉酶的生产方法
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摘要
FIELD: microbiological industry, in particular production of citric acid and acid-resistant α-amylase and glucoamylase enzymes.;SUBSTANCE: claimed method includes starch and maize flour hydrolysis in ratio of 1:7-1:40 with bacterial α-amylase enzyme preparation at elevated temperature and pressure to obtain value of carbon source dextrose equivalent not more than 30; addition of nitrogen organic source and mineral salts such as copper (II), zinc, cobalt (II) sulfates in amount of (0.5-1.0)x10-3, (1.5-3.5)x10-3, (1.5-4.5)x10-3 g/dm3, respectively, iron (II) to starch and maize flour hydrolyzate followed by medium fermentation with acid-forming fungi Aspergillus niger. Than hydrolyzate is diluted with sterile water to obtain fermentable sugar content of 120-140 g/dm3 and carbon and nitrogen organic source in ratio of (50-60):1, respectively. Method of present invention males in possible to convert sugar to citric acid with yield of 79.3-83.1 % and to obtain acid-resistant α-amylase and glucoamylase enzymes having activities of 2.50-3.50 U A-C/cm3 and 80.2-147.4 U Gl-C/cm3, respectively.;EFFECT: method for production of citric acid, α-amylase and glucoamylase with increased yield.;1 tbl, 4 ex
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机译:领域:微生物工业,特别是柠檬酸和耐酸α-淀粉酶和葡糖淀粉酶的生产。物质:所要求保护的方法包括用细菌α-淀粉酶以1:7-1:40的比例水解淀粉和玉米粉在升高的温度和压力下进行酶制备以获得碳源葡萄糖当量的值不超过30;添加(0.5-1.0)x10 -3 Sup>,(1.5-3.5)x10 -3 Sup>,(1.5-4.5)x10 -3 Sup> g / dm 3 Sup>,分别将铁(II)分解为淀粉和玉米粉,然后进行中等发酵与酸形成真菌黑曲霉。然后用无菌水稀释水解产物,得到可发酵糖含量为120-140 g / dm 3 Sup>,碳和氮有机源的比例分别为(50-60):1。本发明的方法可以使雄性将糖转化为柠檬酸,产率为79.3-83.1%,并获得活性为2.50-3.50U AC / cm 3 Sup的抗酸α-淀粉酶和葡糖淀粉酶。 >和80.2-147.4 U G1-C / cm 3 Sup>。效果:增加产量的柠檬酸,α-淀粉酶和葡糖淀粉酶的生产方法; 1 tbl,4 ex
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