首页>
外国专利>
Ultra-high parallel analysis process to analyse nucleic acid chains in which a sample solid is bound and substrate material
Ultra-high parallel analysis process to analyse nucleic acid chains in which a sample solid is bound and substrate material
展开▼
机译:超高并行分析过程,可分析结合了样品固体和基质材料的核酸链
展开▼
页面导航
摘要
著录项
相似文献
摘要
An optical fluorescent process to analyse nucleic acid chains, in which a sample solid is bound with a primer-matrix complex resulting in a cyclic reaction, is new. Marked nucleotides are incorporated in the primer matrix by enzyme reaction, followed by washing of the solid phase. The marked nucleotides are detected and their co-ordinates logged and the signals removed. An optical fluorescent process to analyse nucleic acid chains, in which a sample solid is bound with a primer-matrix complex resulting in a cyclic reaction, is new. Marked nucleotides are incorporated in the primer matrix by enzyme reaction, followed by washing of the solid phase. The marked nucleotides are detected and their co-ordinates logged and the signals removed. The solid phase is then washed and the sequence repeated as necessary. The Nucleic acid chain sequence is then reconstructed using the signals. In a parallel optical analysis process for numerous individual nucleic acid molecules, the surface of the solid phase displays a feature with limited unspecified bond of market components. The non-specific bond has less than 200 events/100 m2.
展开▼