Optical fluorescent ultra-high parallel process to analyse nucleic acid chains in which a sample solid is bound with a primer-matrix complex

摘要

In an optical fluorescent process to analyse nucleic acid chains, a sample solid is bound with a primer-matrix complex resulting in a cyclic reaction. Marked nucleotides are incorporated in the primer matrix by enzyme reaction, followed by washing of the solid phase. The marked nucleotides are detected and their co-ordinates logged and the signals removed. In an optical fluorescent process to analyse nucleic acid chains, a sample solid is bound with a primer-matrix complex resulting in a cyclic reaction. Marked nucleotides are incorporated in the primer matrix by enzyme reaction, followed by washing of the solid phase. The marked nucleotides are detected and their co-ordinates logged and the signals removed. The solid phase is then washed and the sequence repeated as necessary. The Nucleic acid chain sequence is then reconstructed using the signals. In a parallel optical analysis process for numerous individual nucleic acid molecules, the surface of the solid phase displays a feature with limited unspecified bond of market components. The non-specific bond has less than 200 events/100 m2.