MEASURING METHOD OF REACTION RATE COEFFICIENT IN ANALYSIS UTILIZING TOTAL REFLECTION ATTENUATION

摘要

PROBLEM TO BE SOLVED: To provide a measuring method of a reaction rate coefficient in analysis utilizing total reflection attenuation.;SOLUTION: This method for measuring an adsorption rate coefficient and a diffusion coefficient in a reaction between a molecule to be analyzed immobilized on the metal surface and a molecule interacting with the molecule to be analyzed by measuring an angle change of a total reflection attenuation angle by using an analyzer utilizing total reflection attenuation is executed as follows: a plurality of simulation curves of a bonding dissociation reaction are prepared relative to a set of a variable group acquired by changing the adsorption rate coefficient and the diffusion coefficient with a prescribed width respectively; a measurement curve of the bonding dissociation reaction is formed from the measured angle change of the total reflection attenuation angle; the degree of coincidence between the formed measurement curve and the plurality of simulation curves is examined; and an adsorption rate coefficient and a diffusion coefficient used for formation of the simulation curve having the highest degree of coincidence are regarded as the adsorption rate coefficient and the diffusion coefficient in the reaction between the molecule to be analyzed immobilized on the metal surface and the molecule interacting with the molecule to be analyzed.;COPYRIGHT: (C)2007,JPO&INPIT