METHOD FOR DISCRIMINATE TARGET BASE OF DNA AND ALLELE-SPECIFIC PRIMER USED THEREOF

摘要

PPROBLEM TO BE SOLVED: To obtain an allele-specific primer in which when a base directly adjacent to the 3' side of a target SNP base is T and a base adjacent to the base is T, possibility of false positiveness is extremely low and clear SNP discrimination is possible. PSOLUTION: The method for discriminating a target base of DNA is designed such that a 3' terminal base is an SNP corresponding base, the second base from the 3' terminal is G, the third base from the 3' terminal is T, G or C and the base sequence from the fourth base from the 3' terminal to the 5' terminal base is complementary to a base sequence from the target SNP base to a desired base from a base adjacent to three bases to the 3' side. PCOPYRIGHT: (C)2007,JPO&INPIT