Concentration measuring method of the substance to be measured, the sensor chip and its concentration measurement kit for the substance to be measured

摘要

It can be measured in a short period of time the concentration of the substance to be measured a small amount of sample, is to provide a method for measuring the concentration possible to accurately measure the concentration of the substance to be measured amount of sample to be subjected to measurement even if inaccurate. A concentration measuring method for measuring object using a sensor chip which includes the antibody immobilization layer placed on the surface of the optical waveguide layer and an optical waveguide layer, and the enzyme and the sample solution to the antibody immobilization layer of the sensor chip and generates an enzymatic reaction product after fixing the specimen by antigen-antibody reaction by dropwise addition of antibody solution labeled, was added dropwise a color reagent solution, precipitation and color, the enzymatic reaction product to the antibody immobilization layer is the concentration measuring method by total reflection on the antibody immobilization layer light precipitated, was the sensor chip coming from outside and observe changes in the physical quantity of the light is totally reflected.