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Cloning multiple control sequences into chromosomes or into artificial centromeres
Cloning multiple control sequences into chromosomes or into artificial centromeres
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机译:将多个控制序列克隆到染色体或人工着丝粒中
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摘要
Artificially synthesizing 29 homozygous cystic fibrosis core panel controls demonstrates placing multiple homozygous mutant sequences on the same single control DNA sequence to streamline quality control by minimizing extra control assays, time, and costly formatted test materials and testing all controls during every test. Any rare or unavailable reported DNA sequence can be PCR amplified using primer pairs synthesized with the designated mutation or variant sequence with paired adjacent upstream and downstream primers to amplify target sequences in total genomic DNA.
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