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Method of detecting nucleic acid by using DNA microarrays and nucleic acid detection apparatus

机译:利用DNA微阵列检测核酸的方法和核酸检测装置

摘要

A nucleic acid contained in a sample is quantified with high accuracy. The procedure includes the step of allowing the sample containing a specific nucleic acid to interact with a DNA microarray provided with a plurality of nucleic acid probe parts having a nucleic acid probe capable of hybridizing to the specific nucleic acid; the step of monitoring outputs from each of the plurality of nucleic acid probe parts due to hybridization between the nucleic acid probe and the specific nucleic acid to determine normal distribution of time taken to reach a predetermined output value with respect to each of the plurality of nucleic acid probe parts; and the step of quantifying the specific nucleic acid contained in the sample based on a maximum value determined from the normal distribution obtained in the above step.
机译:样品中包含的核酸可以高精度定量。该程序包括以下步骤:使包含特定核酸的样品与DNA微阵列相互作用,所述DNA微阵列具有多个核酸探针部分,所述核酸探针部分具有能够与所述特定核酸杂交的核酸探针;监测由于核酸探针和特定核酸之间的杂交而从多个核酸探针部分中的每个产生的输出的步骤,以确定相对于多个核酸中的每个核酸,达到预定输出值所花费的时间的正态分布酸探针零件根据上述步骤中获得的正态分布确定的最大值,对样品中包含的特定核酸进行定量。

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