Method for producing an acid hialuru00c8nico, host cell of Bacillus, construction of nucleic acid, nucleic acid sequence of isolated, vector expression, recombinant expression vector,Recombinant host cell, method for producing a polipeptu00ecdeo, and polipeptu00ecdeo isolated

摘要

Method for producing an acid hialuru00c8nico, host cell of Bacillus, construction of nucleic acid sequence of nucleic acid isolation, expression vector, recombinant expression vector,Recombinant host cell, method for producing a polipeptu00ecdeo, and polipeptu00ecdeo isolated. The present invention relates to methods for producing hyaluronic acid, comprising: (a) cultivating Bacillus host cell under conditions suitable for the production of hyaluronic acid.In the host cell of Bacillus includes a construction comprising a nucleic acid sequence encoding the hialuronano synthase operavelmente linked to a promoter sequence estranhThe sequence encoding the hialuronano synthase; and (b) recovering the hyaluronic acid from the culture medium.The present invention also relates to a nucleic acid sequence coding a u00f3peron of isolated hialuronano synthase comprising a hialuronano synthase gene and a gene UDP glucose 6Dehydrogenase, and optionally one or more genes selected from the group consisting of a gene UDP glucose pirofosforilase, a gene of UDP nacetilglicosamina pirofosforilase,And a gene of glucose - 6 - phosphate isomerase.The present invention also relates to isolated nucleic acid sequences encodes a UDP - glucose - 6 - desidrocenase, UDP glucose and UDP pirofosforilase, - N - acetilglicosamina pirofosforilase.