DEVELOPMENT OF DIAGNOSTIC KIT FOR PULMONARY AND OTHER EXTRA CENTRAL NERVOUS SYSTEM TUBERCULOSIS USING 30kD ANTIGEN COMPRISING OF Ag 85 COMPLEX

摘要

This invention relates to method for demonstration of 30 kilodalton protein product containing antigen 85 complex comprising of; step 1) coating 100μl specimen in wells of flat bottom microtitre plates and incubation at 37ºC for minutes. 2) Blocking by 0.5% Bovine serum albumin in phosphate buffered saline (PBS) and incubation for 60minutes 3) washing of wells with PBS and addition of Immunoglobulins specific to 30kD protein product and incubation for 45 minutes. 4) washing of well with PBS and addition of anti-immunoglobulins conjugated with HRPO and incubation for 30 minutes.5) A washing with PBS and addition of TMB/H₂O₂ substrate and incubation for 15 minutes for development of colour. 6) Stopping reaction with 2.5 N stop solution and reading absorbance at 450nm.