selection of a strain of bacillus amyloliquefaciencs from ground level hyperproductrice of restriction endonuclease bam type ii appointed: isolation and biochemical characterization of 181 enzyme.

摘要

the present invention relates to the isolation of strains producing bacteriennes new type ii restriction endonucleases. in fact, we have isolated a strain of bacillus amyloliquefaciens from a sample of soil collected in the faculty of sciences of tunis, the university campus.the bacillus strain was found to be hyperproductrice amyloliquefaciencs activity of type ii restriction.in fact, the type ii endonucleases are essential and indispensable in all dna manipulations and can cut inside the sequence recognition or very close to the site and size generant fragments defined is very used. the detection of such enzymes is very simple, but if a great special features.these enzymes do not require more than mg2 + at ph 8.0 buffer (tris hci 10mm). the study of the physicochemical properties of these enzymes, we can determine the optimal functioning of the factors to the enzyme; and use in accordance with the requirements of the experiment and the manipulation is performed.the determination of the site of restriction enzyme, we can, and to compare with other type ii enzymes known to determine the site of recognition; to classify and find out if she's isoschisomere other enzymes, or it's a prototype.