production of potato plants plants - immune to virus and expressing a gene encoding a recombinant antibody fragment directed against a viral protein.

摘要

the present invention relates to the production of potato lignees plants - expressing cdna encoding a recombinant antibody fragment directed against a protein of potato virus y (pvy). the antibody has been characterized only in origin and shows a strong affinite for antigene target.the cdna fragment encoding the scfv or vh of the antibodies were expressed in e. coli and showed a strong activity against the antigene monoclonal antibodies, similar to the original. the fragments showed a reagents against antigens in the tissues infected by the virus.the potato processing was conducted with agrobacterium tumefaciens lba 4404 strain carrying the plasmid pkylx35s2 - scfv / vh. the transformation was carried out on tissues varies different potato varieties.the analysis of plants plants - regenerees was conducted by pcr for dna by rt-pcr for the detection of rna and western blot, and enzyme-linked immunosorbent assay for protein. plants plants - expressing the fragment scfv / mm have been obtained and transferred to a greenhouse inoculees solution concentrated by pvy.they showed resistance to the virus, which resulted in a complete absence of symptoms of virus infection and the viral multiplication. these plants are all plants - montrees immune to the virus.