Method of nucleic acid amplification-based extension moiety (rami) and in vitro transcription.

摘要

The invention relates to an improved method allowing detection standardized fast and responsive, A target nucleic acid of a microorganism PATOGENO or viruses, or gene normal or abnormal, in a sample. The procedure consists of hybridizing a target nucleic acid MULTIPLE PROBES Oligonucleotide nonoverlapping, hybridizing WITH ADJACENT REGIONS IN target nucleic acid, which are called capture probes / amplification and amplification probes, RESPECTIVELY, IN THE PRESENCE OF GRANULES paramagnetic LINED A moiety that binds ligand. By joining a ligand attached to one end of the capture probe / amplification and hybridization SPECIFIED PARTS PROBE TO ADJACENT sequences in the target nucleic acid it is formed A complex comprising the target nucleic acid probes and GRANULES paramagnetic. Such probes can join CONTINUATION together, forming a complex consisting AMPLIFICATION SEQUENCE OF CONTINUOUS COMBINED WITH UNITED Granules, COMPLEX CAN TO ELIMINATE denature nucleic acid and unbound probes. Alternatively, one can EMPLOY AMPLIFICATION PROBES AND SEPARATE CAPTURE, which form PROBES PROBE CIRCULAR OR CONTINUOUS LONG LENGTH, AND MAY detecting or amplifying directly using an amplification technique ADEQUATE, eg .: PCR, RAM O HSAM, FOR SCREENING. Detection of amplification sequence UNIDA, directly or after amplification THEREOF, it indicates the presence of target nucleic acid in a sample. Also provided are methods for detecting SEQUENCE AMPLIFICATION, including a procedure signal amplification hybridization and amplification procedure branching-EXTENSION.