Human monoclonal antibodies against the antigen ia-2 cells of the islets of Langerhans.

摘要

Immortalization of human lymphocytes prediabetic or diabetics with high serum antibody titres to IA-2, culturing the immortalized with growth factors while simultaneously removing lymphocytes factors: they can obte-nerse through the following operational stages human monoclonal antibodies inhibitors, detection of specific human monoclonal antibodies AI-2 in the culture supernatant, cloning the human immortalized cell line producing these antibodies in the presence of cells ALIMEN-Tadoras which contain no cytotoxic T lymphocyte, multiplication of these cells immortalized optionally with the addition of growth factors and isolating the monoclonal antibody produced by this clone.