PLURIPOTENT STEM CELL CLONED FROM SINGLE CELL DERIVED FROM SKELETAL MUSCLE TISSUE

摘要

Disclosed is a method for isolating a pluripotent stem cell which can be differentiated into at least a myocardial cell to regenerate the cardiac muscle, at a high purity. A pluripotent stem cell that can be differentiated into at least a myocardial cell to regenerate the cardiac muscle can be isolated at a high purity by performing the steps of: (i) collecting a skeletal muscle tissue from a mammal and enzymatically treating the skeletal muscle tissue to prepare a cell derived from the skeletal muscle tissue; (ii) cultivating the cell in a culture medium containing an epidermal growth factor and a fibroblast growth factor; and (iii) selecting and separating a colony that is floating in the culture medium.