Pluripotent stem cells cloned from single cells derived from skeletal muscle tissue

摘要

An object of the present invention is to provide a technique for isolating pluripotent stem cells that can differentiate into cardiomyocytes and regenerate myocardium with high purity. (i) collecting skeletal muscle tissue from a mammal and obtaining the skeletal muscle tissue-derived cells by enzymatic treatment of the obtained skeletal muscle tissue, (ii) proliferating the obtained skeletal muscle tissue-derived cells into epithelial cells Culturing in a medium containing a factor and fibroblast growth factor, and (iii) selecting and separating colonies floating in the culture solution to differentiate at least cardiomyocytes and regenerate myocardium Capable pluripotent stem cells can be isolated with high purity.