METHOD OF QUANTITATING NUCLEIC ACIDS BY FLOW CYTOMETRY MICROPARTICLE-BASED ARRAY

摘要

Disclosed is a method of measuring a target nucleic acid species in a sample (e.g., a biological sample) comprising amplifying multiple known concentrations of a standard nucleic acid, wherein the standard nucleic acid comprises the same sequence as the target nucleic acid or a fragment thereof; amplifying the target nucleic acid; hybridizing the amplified target nucleic acid with a population comprising a subset of microparticles to form a mixture, wherein each subset is distinguishable from other subsets based on a detectable parameter and wherein a subset of microparticles is coupled to an oligonucleotide that is complementary to a portion of the target and standard nucleic acids; hybridizing each of the amplified standard nucleic acid samples with a population comprising said subset of microparticles to form mixtures; analyzing the mixtures by flow cytometry, wherein the binding of a target nucleic acid and standard nucleic acid samples to a subset of microparticles is measured based on detection of a label; and determining the quantity of target nucleic acid in the biological sample.