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METHOD OF EVALUATING RNAi ACTIVITY AND miRNA ACTIVITY

机译:评估RNAi活性和miRNA活性的方法

摘要

The present invention provides methods whereby an RNAi activity and a miRNA activity can be evaluated simply and conveniently. The RNAi activity or the miRNA activity is evaluated by supplying a target-expressing molecule, which is a polynucleotide containing at least a target sequence and an expression regulatory region regulating the expression of an RNA containing the target sequence, and a subject nucleic acid molecule to be evaluated on whether or not it has RNAi activity on the RNA containing the target sequence into an expression system in which the target-expressing molecule can express the RNA containing the target sequence.
机译:本发明提供了可以简单且方便地评估RNAi活性和miRNA活性的方法。 RNAi活性或miRNA活性通过提供靶表达分子和主题核酸分子来评估,所述靶表达分子是至少包含靶序列和调节包含靶序列的RNA的表达的表达调节区的多核苷酸。在表达系统中评估其是否对包含靶序列的RNA具有RNAi活性,在该表达系统中靶表达分子可以表达包含靶序列的RNA。

著录项

  • 公开/公告号EP1693450A4

    专利类型

  • 公开/公告日2007-10-31

    原文格式PDF

  • 申请/专利权人 BIO-THINK TANK CO. LTD.;

    申请/专利号EP20040818954

  • 申请日2004-11-18

  • 分类号C12N15/09;C12N15/11;C12Q1/68;G01N33/15;G01N33/50;

  • 国家 EP

  • 入库时间 2022-08-21 20:48:11

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