IDENTIFICATION METHOD AND QUANTIFICATION METHOD FOR PROTEIN SAMPLE USING ELECTROPHORESIS AND MASS SPECTROMETRY

摘要

Disclosed is a method for the analysis of a proteome utilizing electrophoresis which enables the differential display on a single of gel. A method for identification or quantification of a protein sample comprising the steps of (i) providing two protein samples, (ii) modifying the two proteins separately using modification reagents which are only different from each other in the isotope composition, respectively (that is, labeling either of the two protein samples by the isotope labeling method), (iii) mixing the two modified protein samples together, (iv) performing electrophoresis, (v) performing in-gel fragmentation, (vi) concentrating and separating the modified peptides, and (vii) performing mass spectrometry of the resulting peptides.