首页>
中文期刊>
《世界胃肠病学杂志:英文版》
>Identification of differentially expressed proteins between human esophageal immortalized and carcinomatous cell lines by two-dimensional electrophoresis and MALDI-TOF-mass spectrometry
Identification of differentially expressed proteins between human esophageal immortalized and carcinomatous cell lines by two-dimensional electrophoresis and MALDI-TOF-mass spectrometry
AIM:To identify the differentially expressed proteins between the human immortalized seophageal epithelial cell line(SHEE)and the malignant transformed esophageal carcinoma cell line(SHEEC),and to explore new ways for studying esophageal carcinoma associated genes.METHODS:SHEE and SHEECcell lines were used to separate differentially expressed proteins by two-dimensionalelectrophoresis.The siler-stained2-Dgels was scanned with EDAS290 digital camera system and analyzed with the PDQuest6.2Software.Six spots in which the differentially expressed protein was more obviouw were selected and analyzed with matrix-assisted laser desorption/ionization time of flying mass spectrometry(MALDI-TOF-MS).RESULTS:There were107±4.58and115±9.91orotein spots observed in SHEEand SHEEC respectively,and the majority of these spots between the two cell lines matched each other(r=0.772),only a few were expressed differentially.After analyzed by MALDI-TOF-MSand database search for the six differentially expressed proteins,One new protein as well as other five sequence-known proteins including RNPEP-like protein,human rRNA gene upstream sequence binding transcription factor,uracil DNAglycosylase,AnnexinA2 and p300/CBP-associated facto were preliminarily identified.CONCLUSION:These differentially expressed proteins might play an importance role during malignant transformation of SHEEC from SHEE.The identification of these proteins may serve as a new way for studying esophageal carcinoma associated genes.
展开▼