INSULIN-LIKE GROWTH FACTOR-1 IMPROVE VIABILITY IN PORCINE PARTHENOTES AND CLONED EMBRYOS

摘要

An in vitro culture method of porcine parthenotes and cloned embryos is provided to increase cell survival rate, inhibit cell death, and reduce expression of genes inducing cell apoptosis by using insulin-lime growth factor-1(IGF-1), so that they are developed into high quality of blastocysts. The porcine parthenotes and cloned embryos are in vitro cultured in a basic medium(NCSU23) containing 0.4% of bovine serum albumin or glutathione by adding insulin-lime growth factor-1(IGF-1), wherein the amount of insulin-lime growth factor-1(IGF-1) is 10 to 100 ng/ml.