RECOMBINANT YEAST EXPRESSION PLASMID HAVING GSMTX4 GENE AND METHOD FOR MASS-PRODUCING GSMTX4, GRAMMOSTOLA SPATULATA TOXIN PEPTIDE USING THE SAME

摘要

The present invention relates to a Chilean tarantula production method of venom peptide GsMTx4 using a recombinant plasmid for the expression of the yeast containing the GsMTx4 gene and this, AOX (alcohol oxidase) than that specifically express the induced GsMTx4 gene by methanol promoter and It relates to a method to connect to the factor (α-factor) secretion signal sequence and producing the recombinant plasmid for yeast expression, by using this transformed yeast methanol magnetization producing GsMTx4 peptide mass-alpha. When using a recombinant yeast expression system of the invention it is possible to mass production with the GsMTx4 peptide compared to the GsMTx4 peptide of natural, can be used for the treatment of various diseases related to such as a heart attack as a specific inhibitor to block the mechanochemical sensitive ion channels . ; GsMTx4 peptide, Pichia pastoris pass, methanol