首页> 外国专利> GENE OF A PARASITE CAUSING DEATH OF HALOCYNTHIA RORETZI AND METHOD FOR DIAGNOSING THE PARASITE

GENE OF A PARASITE CAUSING DEATH OF HALOCYNTHIA RORETZI AND METHOD FOR DIAGNOSING THE PARASITE

机译:导致食盐综合症死亡的寄生虫基因和诊断寄生虫的方法

摘要

A gene of a parasite causing death of Halocynthia roretzi and a method for diagnosing a parasite are provided to obtain a living organism that causes perishing of Halocynthia roretzi, and to diagnose a parasite by using a part of 18S rDNA fraction causing perishing of Halocynthia roretzi. A method for diagnosing a parasite includes the steps of: separating the entire nucleic acid from Halocynthia roretzi; performing PCR reaction by having the separated nucleic acid as a template and having a front primer and a reverse primer having a partial sequence of 18SrDNA fraction having a nucleotide sequence of sequence number 1 as a primer pair; and checking the size of the product of the PCR reaction by gel electrophoresis and comparing the size of the aforesaids product with the size of the product that is anticipated from the used primer pair.
机译:提供了引起轮状金丝藻死亡的寄生虫的基因和诊断该寄生虫的方法,以获得引起轮状金丝藻灭亡的活生物体,并通过使用一部分导致轮状金丝藻灭亡的18S rDNA组分来诊断寄生虫。诊断寄生虫的方法包括以下步骤:从罗氏海胆中分离出完整的核酸;以分离的核酸为模板,以具有序列号为1的核苷酸序列的18SrDNA部分的部分序列的正向引物和反向引物作为引物对,进行PCR反应。通过凝胶电泳检查PCR反应产物的大小,并将上述产物的大小与从使用的引物对预期的产物的大小进行比较。

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