MUTANTS HAVING A PRODUCING ABILITY OF 4-HYDROXYBUTYRATE AND METHOD FOR PREPARING 4HB USING THE SAME

摘要

Mutants having highly producing ability of 4-hydroxybutyrate(4HB) and a method for producing 4-hydroxybutyrate by using the same mutants are provided to mass produce 4-hydroxybutyrate from highly succinate-producing microorganisms by inserting a 4-hydroxybutyrate-producing gene and deleting a succinate-producing gene. Mutants having highly producing ability of 4-hydroxybutyrate is characterized in that a gene encoding an enzyme converting succinate into 4-hydroxybutyrate is introduced or amplified and gene associated with conversion of succinate semialdehyde into succinate is deleted. The mutant is bacterium, yeast and fungus, wherein the bacterium is selected from Rumen bacterium, Corynebacterium sp., Brevibacterium sp. and Escherichia coli; the enzyme gene converting succinate into 4-hydroxybutyrate is selected from Cat1(succinyl-CoA transferase) gene of SEQ ID NO:1, SucD(succinate semialdehyde dehydrogenase) gene of SEQ ID NO:2, 4hbD(4-hydroxybutyrate dehydrogenase) gene of SEQ ID NO:3 and GHB(4-hydroxybutyrate dehydrogenase) gene of SEQ ID NO:4; and the gene associated with conversion of succinate semialdehyde into succinate is GabD(succinic semialdehyde dehydrogenase) gene of SEQ ID NO:5.