The present invention in advance about from the nucleotide sequence in the defined range specificity to the method and probe for selecting a high probe, even more specifically specific consisting of a nucleic acid for analyzing the genotype of iron Liu Cong virus (Human Papillomavirus, HPV) how to choose a probe and to the probe. In the present invention, in the base sequences within a predetermined range, setting a target nucleotide sequence analysis of the gene group; Setting a range of the probe nucleotide sequence selected from the base sequence group; But selecting the candidate probe within the sequence range, selecting a candidate probe length of 20 to 50 mer of the candidate probe; Comprising, among the candidate probe, the melting temperature between the target nucleic acid and probe for the candidate probe candidates are selected for the candidate probe 50 to 80 ℃; Among the selected probe candidates, from each of the base sequence of the analyte nucleotide sequence group and the other nucleotide sequence other than the nucleotide sequence of the target nucleic acid, select a temperature not more than the probe candidates of the melting temperature with the selected probe candidates, hybridization conditions the step of; And specificity comprising the step of the melting temperature of the secondary structure from the selected probe candidates, select 5 to 10 ℃ lower temperature and higher temperature of the probe candidates than the temperature of the hybridization conditions (specificity) It provides a high probe selection method and the probe. ; Human papilloma virus (HPV), a nucleic acid, the probe nucleotide sequence, the melting temperature
展开▼
