Method Selecting Highly Specific Probes For HPV Genotype Analysis and the Probes Thereof

摘要

A method for selecting a highly specific probe among a predetermined range of nucleotide sequences comprises:setting a group of nucleotide sequences among the predetermined range of nucleotide sequences; setting a range of nucleotide sequences in the group of nucleotide sequences; selecting first candidate probes having a certain length within the range of nucleotide sequences; selecting second candidate probes whose melting temperature with target nucleic acids for the first candidate probes is in an appropriate range; selecting third candidate probes whose melting temperature with a specific set of nucleotide sequences is lower than a hybridization temperature; and selecting fourth candidate probes, wherein a secondary structure of each fourth candidate probe has a melting temperature lower than the hybridization temperature by approximately 5° C. to approximately 10° C. and higher than a temperature that is lower than the hybridization temperature by approximately 5° C. to approximately 10° C.