1 2 A Method for Mass Production of Recombinant Trypsin by Employing Regulatory Genes sgtR1 and sgtR2 from Streptomyces griseus

摘要

A method for mass production of recombinant trypsin by using regulatory genes sgtR1 and sgtR2 derived from Streptomyces griseus is provided to improve production yield of trypsin compared to the conventional methods by using a transformant. A trypsin recombinant expression vector for transforming prokaryotes contains sprT gene derived from Streptomyces griseus, and sgtR1 and sgtR2 genes derived from Streptomyces griseus, wherein the trypsin recombinant expression vector is pWHM3-TR1, pWHM3-TR2 or pWHM3-TR1R2. The method for mass production of recombinant trypsin comprises the steps of: constructing the trypsin recombinant expression vector; producing transformed Streptomyces griseus with the trypsin recombinant expression vector; and culturing the transformed Streptomyces griseus and harvesting trypsin from the cultured medium.