Preparation of the Streptomyces strains producing alpha-glucosidase inhibitors transformed by the recombinant vectors harboring the salbostatin biosynthesis gene cluster, and mass-production method of these inhibitors

摘要

The invention alpha Using Salvo statin biosynthetic genes - relates to a mass production method of a-glucosidase inhibitor, Salvo statin producing strain of Streptomyces albus (Streptomyces albus) - glucosidase inhibitor producing the production strain and alpha genus Streptomyces having the glucosidase inhibitory activity - derived from KCTC 9015, and by introducing a part of the base sequence of about 17kb Salvo statin biosynthetic genes of which comprises the 14 enzyme coding gene region in the salH to salU alpha It relates to a method for producing a transformant of a strain derived. ; According to the invention, from transformant prepared above alpha-glucosidase Bali yen it can produce large amounts of the amine or Bali olamine to inhibit, replacing the starting material of the existing anti-diabetic agent, and inexpensively producing an anti-diabetic agent It can have excellent effect. ; Streptomyces albus, Salvo statins, enamine Bali, Bali come amine