Method of preparing ?-Indoline-2-carboxylic acid and S-Indoline-2-carboxylic acid methyl ester using a hydrolytic enzyme

摘要

The present invention (S) using a hydrolase - relates to a method for producing indoline-2-carboxylic acid and its methyl ester compound, more specifically, the Sabina (Savinase), alkaline agent (Alcalase), Novo luggage 243 (Novozym 243), Eberhard cyclase (Everlase), Esper cyclase (Esperlase), protease 7 (protease 7) and acylase (S) using selected hydrolytic enzymes from the group consisting of (acylase) - indoline- It relates to a process for preparing 2-carboxylic acid and its methyl ester. ; Manufacturing method according to the present invention, the manufacturing process more than 99% ee optical purity while still simplified, because the optical purity and yield, while using inexpensive industrial enzyme is superior (S) - indoline-2-carboxylic acid methyl ester and his It can be provided and is advantageous because it appears economically greater efficiency compared to the conventional development process by extremely useful industrially available is a practical approach. ; Hydrolase, racemic indoline-2-carboxylic acid methyl ester, (S) - indoline-2-carboxylic acid methyl ester, (S) - indoline-2-carboxylic acid