Semi-continuous biosynthesis of biomolecule by microorganisms in fermentation container, comprises supplying a culture medium into the container, and inoculating the culture medium by aseptic supply of the microorganisms

摘要

The Semi-continuous biosynthesis of biomolecule by bacteria, yeast and cell line in fermentation container, comprises supplying (a) a culture medium into the fermentation containers, inoculating (c) the culture medium through aseptic supply of the microorganism, subjecting the fermentation container to a heated and ultrasonically irradiated fermentation bath containing dissolved oxygen or oxygen mixture, and removing (f) the containers from the bath. The oxygen mixture contains carbon dioxide, methane and/or hydrogen sulfide with an inert gas, and the bath contains a substance. The Semi-continuous biosynthesis of biomolecule by bacteria, yeast and cell line in fermentation containers, comprises supplying (a) a culture medium into the fermentation containers, inoculating (c) the culture medium through aseptic supply of the microorganism, subjecting the fermentation container to a heated and ultrasonically irradiated fermentation bath containing dissolved oxygen or oxygen mixture, and removing (f) the containers from the bath. The oxygen mixture contains carbon dioxide, methane and/or hydrogen sulfide with an inert gas, and the bath contains a substance. The containers are closed (b) in such a way that a gas transfer with the environment takes place by a gas permeable outer wall of the containers, and are sterilized. The gas or the gas mixture serves to register the oxygen for an aerobic fermentation and/or to discharge the oxygen in an aerobic fermentation formed fluid by-products. The fermentation bath is injected into the containers at a pressure equally large as or larger than the atmospheric pressure. The volume of each container is at most 1000 ml and/or the ratio of external surface of each container to its internal volume is 0.5 cm 2/ml.